Integrated microbiome and metabolome unveiled the effect of carbonate buffer mixture alleviating subacute rumen acidosis - Mediated endometritis in dairy goats.

Carbonate buffer supplementation alleviates SARA-induced endometritis through microbiota and metabolite modulation.

This study investigated the therapeutic potential of carbonate buffer mixture (CBM) supplementation in alleviating subacute ruminal acidosis (SARA)-induced endometritis in dairy goats. Thirty lactating dairy goats were subjected to a high-concentrate diet to induce SARA, then treated with CBM. Using integrated 16S rRNA sequencing and untargeted metabolomics, researchers characterized changes in rumen and uterine microbiota alongside serum metabolite profiles. CBM supplementation effectively reversed SARA, reducing LPS levels, neutrophil infiltration, and inflammatory cytokine concentrations in endometrial tissue while enhancing tight junction protein expression. The treatment restored dysbiotic uterine microbiota by increasing beneficial taxa (Lachnospiraceae, Prevotella, Ruminococcus) and modulating rumen bacterial populations. Metabolomic analysis revealed CBM normalized elevated serum creatinine, L-methionine, and L-tyrosine while restoring decreased tryptamine, gabapentin, and sphinganine levels. The findings establish a mechanistic link between ruminal health and uterine immunity, demonstrating that rumen pH stabilization and microbiota restoration directly influence systemic metabolic profiles and endometrial health.

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