Dot-blot ELISA assay demonstrates strong agreement with hemagglutination inhibition for the detection of canine parvovirus antibodies in sera of healthy, vaccinated blood-donor dogs.

Dot-blot ELISA reliably estimates CPV titers for rapid blood-donor screening.

This study evaluated the agreement between dot-blot ELISA and hemagglutination inhibition (HI) assays for detecting canine parvovirus (CPV) antibodies in sera from 100 healthy community blood-donor dogs. Researchers compared results from two state veterinary diagnostic laboratories using HI methodology against a dot-blot ELISA assay. The dot-blot ELISA demonstrated high sensitivity (96-97%) and strong agreement with HI titers, with 92-96% of samples falling within a 2-fold dilution difference. Spearman correlation coefficients were strong (ρ = 0.72-0.92). Among 43 dogs with vaccination history, 93% possessed protective CPV titers, with the lowest titers in recently vaccinated animals (within 1 year). The findings support dot-blot ELISA as a reliable, rapid alternative to reference HI assays for estimating CPV antibody titers. This is particularly valuable for in-house screening of blood-donor dogs and timely selection of high-titer plasma for passive immunotherapy in critically ill parvoviral patients.

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