Effect of Fluorescence Photobiomodulation on Canine Progenitor Epidermal Keratinocytes With and Without Staphylococcus pseudintermedius Colonisation.

FPB shows mild anti-inflammatory effects in vitro but lacks demonstrated antimicrobial efficacy.

This in vitro study investigated fluorescence photobiomodulation (FPB) as a potential therapeutic intervention for canine dermatitis by examining its effects on canine progenitor epidermal keratinocytes (CPEK) with and without Staphylococcus pseudintermedius colonization. Keratinocytes were exposed to two 2-minute FPB treatments, and supernatants were analyzed at multiple timepoints (0, 1, 6, and 24 hours) for inflammatory cytokines, host defense peptides, and bacterial colony counts. Cell viability was assessed using cytotoxicity assays. Results demonstrated a mild but statistically significant decrease in IP-10 fluorescence intensity at 6 hours post-FPB in colonized keratinocytes (p = 0.04). However, no significant differences were observed in other cytokines, host defense peptides, bacterial colony counts, or cell cytotoxicity. The findings suggest FPB may possess mild anti-inflammatory properties in vitro, though the clinical relevance remains uncertain given the limited effects observed and lack of antimicrobial activity against S. pseudintermedius.

This summary was distilled by AI and may occasionally misinterpret data. Confirm critical details with the primary literature before clinical application.