Establishing a standardized biorepository across 3 clinical and translational science center-affiliated veterinary institutions is feasible and productive.

Multi-institutional veterinary biobanking is feasible, producing high-quality, clinically annotated biospecimens suitable for neurodegenerative biomarker research.

This prospective multi-institutional feasibility study (2022–2025) evaluated the development of a standardized biological fluid biorepository across three Clinical and Translational Science Center-affiliated veterinary institutions. Faculty surveys revealed strong support for centralized biobanking, with 55% of 170 respondents favoring a combined institutional and investigator funding model. Over 2,500 biological specimens—including serum, CSF, and urine—were collected from dogs and cats presenting to Neurology and Neurosurgery Services at each institution. A shared database platform (Freezerworks) was implemented to standardize sample annotation with clinical metadata and track storage locations across sites. Quality control assessments of selected serum (n=12), CSF (n=12), and urine (n=9) samples demonstrated consistent quality across all three institutions. Furthermore, putative neurodegenerative disease biomarkers measured via ELISA and single-molecule array technology yielded robust, discriminatory concentrations in serum samples from dogs with divergent neurological diagnoses, confirming fitness for purpose. The study demonstrates that multi-institutional veterinary biobanking is both operationally feasible and scientifically productive. A shared biospecimen registry with reciprocal investigator access could significantly advance translational and comparative neurology research by enabling well-powered, rigorous multicenter studies. This infrastructure has important implications for accelerating biomarker discovery and validation in veterinary neurology, potentially benefiting both animal patients and human neurological disease research through the One Health framework.

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