BLOS1 overexpression enhances goat immune response to Brucella LPS through augmented autophagy with associated gut microbiota remodeling.
Wang C, Liu X, Wan S, Xie F, Dai J, Chen W, Qu L, Zhang L, Li N, Du X, Zhu H, Hua J · Veterinary Journal · 19 May 2026
BLOS1 overexpression enhances Brucella resistance via autophagy and microbiota remodeling.
This study demonstrates that BLOS1 overexpression in goats enhances innate immune responses against Brucella infection through multiple mechanisms. Macrophages from transgenic goats overexpressing BLOS1 showed enhanced activation of NF-κB and TLR4 signaling pathways upon Brucella LPS stimulation, resulting in increased TNF-α secretion. Critically, BLOS1 overexpression significantly upregulated autophagy-related genes (LAMP2 and BECN1), improving autophagic flux and bacterial clearance capacity. Beyond cellular immunity, BLOS1 overexpression remodeled the intestinal microbiota composition, enriching pathways associated with membrane transport and cellular motility while reducing abundance of virulence factors and opportunistic pathogens. These microbiota changes contributed to improved intestinal immune homeostasis. The findings suggest BLOS1 serves as a multi-target enhancer of anti-Brucella immunity, operating at both cellular and microbial ecosystem levels, with potential applications in developing disease-resistant livestock.
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