Evaluation of point-of-care versus laboratory antiglobulin-enhanced major crossmatch testing in cats.

Point-of-care gel crossmatch shows moderate agreement with laboratory testing but may miss incompatibilities.

This prospective study evaluated the agreement between two antiglobulin-enhanced major crossmatch methods in cats requiring blood transfusion. Ninety-five unique donor-recipient pairings were tested using both a laboratory-based tube crossmatch method (reference standard) and a point-of-care gel column crossmatch method performed in-clinic. The laboratory method identified 21 incompatible pairings while the point-of-care method identified 18, with 13 pairings incompatible on both tests. Statistical analysis yielded a kappa agreement of 0.581, indicating moderate agreement between methods. Overall percent agreement was 86.3%, with positive percent agreement of 93.2% and negative percent agreement of 61.9%. While the point-of-care gel crossmatch test demonstrated clinically acceptable agreement with the reference laboratory tube method, the lower negative percent agreement (61.9%) suggests it may miss some incompatible pairings. The authors acknowledge that evaluation of larger numbers of incompatible samples would strengthen conclusions regarding test reliability.

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