Establishment of a Canine Corneal Endothelial Cell Line Using Simian Virus 40 Induction.

Immortalized canine corneal endothelial cells provide a sustainable research tool maintaining primary cell properties.

This study successfully established an immortalized canine corneal endothelial cell (CEC) line using simian virus 40 large T antigen transfection to address the scarcity of primary CECs for veterinary research. Corneal endothelial cells were isolated from canine eyeballs, cultured, and transfected for immortalization. The immortalized cells demonstrated characteristic small, cobblestone morphology and expressed important CEC markers including zonula occludens-1 and sodium-potassium adenosine triphosphatase. The cells maintained robust proliferative capacity through passages 25-30, preserving morphological characteristics throughout extended culturing. This represents a significant advancement in veterinary ophthalmology research infrastructure, providing a renewable source of canine CECs that maintain functional properties of primary cells. The established cell line offers valuable applications for optimizing culture conditions, evaluating therapeutic agents, and developing cell-based therapies specific to canine corneal endothelial pathology.

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